cd137l his  (R&D Systems)

Journal: bioRxiv

Article Title: Identification of Novel Extracellular Vesicles Scaffold Proteins for Versatile Cargo Engineering

doi: 10.64898/2026.01.04.697584

Figure Lengend Snippet: (A) Schematic diagram showing the EVs engineering of Cas9 mediated by RFTN1-N15. (B) Cas9 reporter assay analysis at 48 hours post EVs treatment. Scale bar: 20 μm. (C) Cas9 editing efficiency analysis as quantified by EGFP positive cells ratio from flow cytometry analysis. N=2. Values are plotted as mean ± SD. (D) Schematic diagram showing the EVs engineering of the minimal RISC comlpex mediated by RFTN1-N15. (E) EGFP silencing analysis at 48 hours post EVs treatment. Scale bar: 20 μm. (F) EGFP silencing efficiency analysis as quantified by EGFP mean fluorescence intensity from flow cytometry analysis. N=2. Values are plotted as mean ± SD. (G) Schematic diagram showing the EVs engineering of FCU1 mediated by RFTN1-N15. (H) Cellular toxicity assay analysis at 48 hours post EVs treatment. Scale bar: 100 μm. (I) Cell viability as quantified by CCK8 assay. N=2. Values are plotted as mean ± SD. (J) Schematic diagram showing the EVs engineering of type II transmembrane proteins mediated by RFTN1-N15. (K) Western blot analysis for engineered TMPRSS2 EVs. (L) Western blot analysis for engineered 4-1BBL EVs.

Article Snippet: The following antibodies were used: CD9 (abcam, AB263019), CD81 (Cell Signaling Technology, 56039S), Calnexin (abcam, ab22595), CD63 (Cell Signaling Technology, 2897), Cre recombinase (Cell Signaling Technology, 15036), TSG101 (abcam, ab125011), TMPRSS2 (Cell Signaling Technology, 39665), 4-1BBL (Cell Signaling Technology, 59127), Arginase1 (Cell Signaling Technology, 93668), iNOS (abcam, ab178945), HRP Goat Anti-Rabbit IgG (H+L) (ABclonal, AS014), HRP Goat Anti-Mouse IgG (H+L) (ABclonal, AS003).

Techniques: Reporter Assay, Flow Cytometry, Fluorescence, CCK-8 Assay, Western Blot

Journal: Molecular Therapy Oncology

Article Title: Targeted expansion of cytotoxic T cells using IL-12 and CD137L supplementation enhances antitumor efficacy

doi: 10.1016/j.omton.2025.200996

Figure Lengend Snippet: Comparison of Vδ2 T cell frequency and cytotoxic markers using different protocols in 10-day culture expansions (A) Scheme of the culture conditions tested and concentrations of the different compounds. Frequency and representative histograms from four conditions of cytotoxic Vδ2 T cells expressing (B) granzyme B and (C) IFN-γ. Frequency of Vδ2 T cells producing GzmB and expressing (D) CD16 or (E) NKG2A. Horizontal dash lines represent the mean of all combination experiments. Representative histograms show the comparison between ALN+IL-2 and conditions receiving CD137L. p values represent the highest statistical difference among the different tested conditions (ANOVA).

Article Snippet: Vitamin C (2-phospho-L-ascorbic acid trisodium salt, MilliporeSigma) was used at 50 μg/mL, CD277 (clone 20.1, BT3.1, Thermo Fisher Scientific) at 0.5 μg/mL, CD6 (clone UMCD6, MilliporeSigma) at 10 μg/mL, and CD137L (AdipoGen Life Sciences) at 10 μg/mL.

Techniques: Comparison, Expressing

Journal: Molecular Therapy Oncology

Article Title: Targeted expansion of cytotoxic T cells using IL-12 and CD137L supplementation enhances antitumor efficacy

doi: 10.1016/j.omton.2025.200996

Figure Lengend Snippet: Increased frequency of cytotoxic Vδ2 T cells expanded with ALN, IL-2+IL-12 combined with CD137L (A) Scheme of the expansion protocol. (B) Frequency and (C) absolute numbers of Vδ2 T cells before (basal) and after 18–19 days of expansion with ALN and IL-2. (D) Correlation between basal and expanded Vδ2 T cell frequencies. Spearman correlation test. Comparison of cytotoxic markers (E) GzmB and (F) CD16, (G) immune checkpoint NKG2A, and distribution of GzmB within (H) CD16 + or (I) NKG2A + Vδ2 + cells upon expansion with ALN+IL-2 compared to ALN+IL-2+IL-12+CD137L. (J) Distribution of GzmB production in CD16/NKG2A Vδ2 T cell populations. FC, fold change expansion. Wilcoxon matched-pairs signed-rank tests. ns, not significant.

Article Snippet: Vitamin C (2-phospho-L-ascorbic acid trisodium salt, MilliporeSigma) was used at 50 μg/mL, CD277 (clone 20.1, BT3.1, Thermo Fisher Scientific) at 0.5 μg/mL, CD6 (clone UMCD6, MilliporeSigma) at 10 μg/mL, and CD137L (AdipoGen Life Sciences) at 10 μg/mL.

Techniques: Comparison

Journal: Molecular Therapy Oncology

Article Title: Targeted expansion of cytotoxic T cells using IL-12 and CD137L supplementation enhances antitumor efficacy

doi: 10.1016/j.omton.2025.200996

Figure Lengend Snippet: Increased cytolytic function of Vδ2 T cells expanded with an optimized protocol Comparison of the cytotoxic function of Vδ2 T cells expanded with either ALN+IL-2 or ALN+IL-2+IL-12+CD137L in a non-radioactive cytotoxic assay, against cancer cell lines (A) K562, (B) H1299, (C) A2780, (D) MCF7, and (E) T47D. (F) Difference of cancer cell lines killing between Vδ2 T cells expanded with either protocol. Wilcoxon matched-pairs signed-rank tests. ns, not significant.

Article Snippet: Vitamin C (2-phospho-L-ascorbic acid trisodium salt, MilliporeSigma) was used at 50 μg/mL, CD277 (clone 20.1, BT3.1, Thermo Fisher Scientific) at 0.5 μg/mL, CD6 (clone UMCD6, MilliporeSigma) at 10 μg/mL, and CD137L (AdipoGen Life Sciences) at 10 μg/mL.

Techniques: Comparison

Journal: Molecular Therapy Oncology

Article Title: Targeted expansion of cytotoxic T cells using IL-12 and CD137L supplementation enhances antitumor efficacy

doi: 10.1016/j.omton.2025.200996

Figure Lengend Snippet: Increased Vδ2 T cell cytolytic function against cancer cell lines in overnight cocultures (A) Comparison of cytotoxic function by flow cytometry against cancer cell lines K562, H1299, A2780, MCF7, and T47D and (B) difference in cytotoxicity of Vδ2 T cells from eight donors expanded with either ALN+IL-2 or ALN+IL-2+IL-12+CD137L. Intracellular production of GzmB in (C) total (D) CD16 + and (E) NKG2A + Vδ2 T cells and frequency of (F) CD16 and (G) NKG2A expression upon coculture of different cancer cell lines with Vδ2 T cells expanded with ALN+IL-2 (dark gray) or ALN+IL-2+IL-12+CD137L (light gray). Wilcoxon matched-pairs signed-rank tests. ns, not significant. Circles represent cancer cell lines cultured alone, and cocultures with Vδ2 T cells expanded with IL-2 are shown with triangles and those expanded with IL-2+IL-12+CD137L with squares. (H) Production of cytolytic molecules in the supernatant upon coculture.

Article Snippet: Vitamin C (2-phospho-L-ascorbic acid trisodium salt, MilliporeSigma) was used at 50 μg/mL, CD277 (clone 20.1, BT3.1, Thermo Fisher Scientific) at 0.5 μg/mL, CD6 (clone UMCD6, MilliporeSigma) at 10 μg/mL, and CD137L (AdipoGen Life Sciences) at 10 μg/mL.

Techniques: Comparison, Flow Cytometry, Expressing, Cell Culture